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Reconstitution Guide
Materials Needed
- Lyophilized peptide vial
- Bacteriostatic water (BAC water) – 0.9% benzyl alcohol
- Sterile syringe (1mL or 3mL insulin syringe)
- Alcohol swabs
- Clean work surface
Step-by-Step Instructions
- Prepare your workspace. Clean your work surface and wash your hands thoroughly.
- Allow vial to reach room temperature. Remove the peptide vial from storage and let it warm to room temperature (15-20 minutes).
- Clean the vial stoppers. Wipe the rubber stopper of both the peptide vial and BAC water vial with an alcohol swab. Allow to dry.
- Draw bacteriostatic water. Using a sterile syringe, draw up your desired amount of BAC water (see calculator below).
- Inject slowly along the vial wall. Insert the needle into the peptide vial at an angle and slowly release the water down the inside wall of the vial – NOT directly onto the powder.
- Allow to dissolve. Let the water gently saturate the powder. Do not shake. You can gently swirl the vial if needed.
- Inspect the solution. The peptide should fully dissolve into a clear solution. If cloudy or particles remain, continue gentle swirling.
Concentration Calculator
For a 10mg peptide vial, adding different amounts of BAC water yields:
| BAC Water | Concentration | 0.1mL = |
|---|---|---|
| 1.0 mL | 10 mg/mL | 1.0 mg |
| 2.0 mL | 5 mg/mL | 0.5 mg |
| 2.5 mL | 4 mg/mL | 0.4 mg |
| 4.0 mL | 2.5 mg/mL | 0.25 mg |
⚠️ Important Notes
- Never shake peptide solutions vigorously – this can denature the peptide
- Use bacteriostatic water, not sterile water, for multi-use vials
- Store reconstituted peptides refrigerated at 2-8°C
- Use within 28 days of reconstitution for optimal stability